Cal Tech - SDS Page Gel Electrophoresis
Introductory paragraphs and figure are a useful orientation. Pay attention to the description of the role of SDS (sodium dodecyl sulfate).
Davidson College - SDS-PAGE (PolyAcrylamide Gel Electrophoresis)Good, basic tutorial.
Rice University - Preparing Protein Samples for ElectrophoresisWhat is the purpose of the SDS? What is the purpose of the β-mercaptoethanol? What would be the result of running a gel without SDS (native gel)? What about without the mercaptoethanol (non-reducing)?